Oral allergy syndrome (OAS) involves the rapid onset of symptoms induced by food allergens in the oral and pharyngeal mucosa, including itching and/or angioedema of the lip, tongue, palate, and throat. In conclusion, we developed mouse model of OAS to identify IgE cross-reactive pollen and food allergens, which will help understand the pathogenesis of OAS/PFAS.įood allergy is defined as a phenomenon in which adverse reactions are caused by allergen-specific immunological mechanisms following exposure to a given food ( 1– 5). These results indicate IgE cross-reactivity of ragweed pollen with black pepper and fennel. Importantly, analysis of murine model of OAS showed that the injection of black pepper or fennel extract induced apparent oral allergic responses in ragweed pollen-sensitized mice. We confirmed that the levels of black pepper-specific IgE and fennel-specific IgE were significantly higher in the serum from ragweed pollen-sensitized mice than in the serum from non-sensitized control mice.
Protein microarray analysis showed that serum IgE from mice intraperitoneally sensitized with ragweed pollen, one of the major pollens causing pollinosis, bound highly to protein extracts from several edible plants including black peppercorn and fennel. Therefore, we used this model of OAS to examine IgE cross-reactive allergens in vivo. In addition, the degree of mast cell degranulation in the oral cavity, reflecting the severity of oral allergic responses, can be estimated by measuring the amount of extravasated dye in the skin.
#Black pepper allergy cross reactivity skin#
Interestingly, when the food allergen ovalbumin (OVA) was injected inside the lower lip of mice with high levels of OVA-specific IgE followed by an intravenous injection of the Evans blue dye, we found immediate dye extravasation in the skin of the neck in a mast cell-dependent manner. We focused on lip angioedema, which is one of the most common symptoms of OAS, and confirmed that mast cells reside in the tissues inside the lower lip of the mice.
In the present study, we developed a method to comprehensively identify cross-reactive allergens by using murine model of OAS and protein microarray technology. However, we need to more precisely understand the underlying pathogenesis of OAS/PFAS. Pollen food allergy syndrome (PFAS), a pollinosis-associated OAS, is caused by cross-reactivity between food and pollen allergens. Oral allergy syndrome (OAS) is an IgE-mediated immediate food allergy that is localized to the oral mucosa.
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